Emfret M023-2說明書
Cat.No.:XX | M023-2 |
抗原： | 整合素αIIbbeta3（GPIIb / IIIa，CD41 / CD61） |
描述： | PE綴合的JON / A抗體選擇性結(jié)合小鼠整聯(lián)蛋白αIIbβ3的高親和力構(gòu)象，小鼠整合素αIIbβ3是許多配體的血小板/巨核細(xì)胞特異性受體，包括血管性血友病因子（vWf），纖維蛋白原，纖維連接蛋白和玻連蛋白。 。 |
克隆： | JON / A. |
同種型： | 大鼠IgG2b |
形成： | PE |
應(yīng)用： | FC（活動表格） |
尺寸： | 1.5毫升; 300次測試 |
價錢： | 499.00美元 |
特異性：PE結(jié)合的jon/A抗體選擇性地結(jié)合小鼠整合素aIIb 3 1(GPIIbIIIa，CD 41/CD 61)的高親和力構(gòu)象，這是一種由135 kDa ai組成的糖蛋白復(fù)合物。 IB鏈和90 kDa b3鏈。整合素aIIbb3是纖維蛋白原、血管性血友病因子、纖維連接蛋白和維甲酸的血小板受體，介導(dǎo)血小板粘附和聚集。A型 整合素aIIbb3中的陽離子依賴性構(gòu)象變化，因此喬恩/A-PE的結(jié)合依賴于細(xì)胞外游離鈣。
制備和保存：用蛋白G-Sepharose層析法從雜交瘤細(xì)胞培養(yǎng)上清液中純化抗體.該抗體與R-生理紅蛋白(PE)在宜條件下結(jié)合。 尼迪奇。將產(chǎn)品保存在4°C未稀釋，避免長時間暴露在光線下。從裝運之日起穩(wěn)定一年。別凍著。
用途：本抗體制備方法適用于流式細(xì)胞術(shù)應(yīng)用：推薦用5μl染色~106血小板或~0.5x 106細(xì)胞，體積為25μl泰氏-Hepes緩沖液。 Ng 1毫米CaCl 2室溫下孵育15 min，加入400μmol/L的PBS停止反應(yīng)，30 min內(nèi)對樣品進(jìn)行分析。請注意孵化時間、緩沖條件的變化。 S，或抗體濃度可能影響瓊恩/A-PE的結(jié)合.
警告： Sodium azide是一種可逆的氧化代謝抑制劑；因此，含有這種防腐劑的抗體制劑不得用于細(xì)胞培養(yǎng)或動物體內(nèi)注射。 。Sodium azide可以通過洗滌染色細(xì)胞或平板結(jié)合抗體或在無Sodium azide緩沖液中透析可溶性抗體來去除。
emfret授權(quán)書：
EMFRET Analytics—專注脈管系統(tǒng)抗體
德國EMFRET公司專注心血管和血液系統(tǒng)研究用抗體研發(fā)和生產(chǎn)，供應(yīng)的抗體包括清除血液中血小板、流式細(xì)胞術(shù)鑒定分析小鼠血小板表面糖蛋白，EMFRET公司的抗體不僅適用于流失分析、WB檢測，還可用于免疫組化/免疫熒光及免疫共沉淀檢測。
Cat.No. | Antigen | Clone | Isotype | Form | Applications | Size |
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C301 | Antibodies for Mouse Plaet Depletion | polyclonal non-immune rat immunoglobulins | Rat IgG | purif. | negative control for #R300 | 0.5 mg |
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D200 | Two-Color Analysis of Mouse Plaet Activation | Wug.E9 JON/A | Rat IgG2b/IgG1 | FITC PE | flow cytometry | 2 x 1.0 ml; 200 tests |
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M011-0 | GPVI | JAQ1 | Rat IgG2a | purif. | IP, IHC, WB | 0.1 mg |
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M011-1 | GPVI | JAQ1 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
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M021-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.H4 | Rat IgG2b | purif. | IP, IHC, block | 0.5 mg |
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M021-1 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.H4 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
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M022-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.A1 | Rat IgG1 | purif. | IP, IHC | 0.5 mg |
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M023-2 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | JON/A | Rat IgG2b | PE | FC (activ. form) | 1.5 ml; 300 tests |
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M024-0 | Integrin alphaIIb (GPIIb, CD41) | MwReg30 | Rat IgG1 | purif. | IP, IHC, generation of Fab fragments | 6 mg |
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M025-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | purif. | IP, IHC | 0.5 mg |
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M025-1 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
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M025-2 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | PE | FC, IHC | 1.5 ml; 300 tests |
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M025-3 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | DyLight 649 | FC, IHC | 1.5 ml; 300 tests |
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M030-0 | Integrin beta3 chain (GPIIIa, CD61) | Luc.A5 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg |
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M031-0 | Integrin beta3 chain (GPIIIa, CD61) | Luc.H11 | Rat IgG2b | purif. | IP, IHC, WB | 0.5 mg |
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M031-1 | Integrin beta3 chain (GPIIIa, CD61) | Luc.H11 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
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M040-0 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | purif. | IP, IHC | 0.5 mg |
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M040-1 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
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M040-2 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | PE | FC, IHC | 1.5 ml; 300 tests |
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M040-3 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | DyLight 649 | FC, IHC | 1.5 ml; 300 tests |
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M041-0 | GPIbalpha (CD42b) | Xia.H10 | Rat IgG2a | purif. | FC, IHC | 0.5 mg |
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M041-1 | GPIbalpha (CD42b) | Xia.H10 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
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M042-0 | GPIbalpha (CD42b) | Xia.G7 | Rat IgG1 | purif. | IP, IHC, WB | 0.5 mg |
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M042-1 | GPIbalpha (CD42b) | Xia.G7 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
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M043-0 | GPIbalpha (CD42b) | Xia.B2 | Rat IgG2a | purif. | IP, IHC, block | 0.5 mg |
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M043-1 | GPIbalpha (CD42b) | Xia.B2 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
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M050-0 | GPIbbeta (CD42c) | Xia.C3 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg |
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M050-1 | GPIbbeta (CD42c) | Xia.C3 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
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M051-0 | GPIX (CD42a) | Xia.B4 | Rat IgG1 | purif. | IP, IHC | 0.5 mg |
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M051-1 | GPIX (CD42a) | Xia.B4 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
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M060-0 | GPV (CD42d) | Gon.C2 | Rat IgG1 | purif. | IP, IHC | 0.5 mg |
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M060-1 | GPV (CD42d) | Gon.C2 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
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M061-1 | GPV (CD42d) | Gon.G6 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
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M070-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.G4 | Rat IgG2b | purif. | IP, IHC, block | 0.5 mg |
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M070-1 | Integrin alpha2 chain (CD49b, GPIa) | Sam.G4 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
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M071-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1 | Rat IgG2b | purif. | IP, IHC | 0.5 mg |
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M071-1 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
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M075-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1, Sam.G4, Sam.H9 | Rat IgG2b/IgG1 | purif. | IP, IHC | 0.5 mg |
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M080-0 | Integrin alpha5 chain (CD49e) | Tap.A12 | Rat IgG1 | purif. | IP, IHC | 0.5 mg |
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M080-1 | Integrin alpha5 chain (CD49e) | Tap.A12 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
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M110-0 | CD9 | Nyn.H3 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg |
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M110-1 | CD9 | Nyn.H3 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
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M120-0 | CD31 (PECAM-1) | Pec.H3 | Rat IgG1 | purif. | IP, IHC, WB | 0.5 mg |
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M130-0 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | purif. | IP, IHC | 0.5 mg |
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M130-1 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
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M130-2 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | PE | FC, IHC | 1.5 ml; 300 tests |
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P140-1 | fibrinogen | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.5 ml; 300 tests |
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P150-1 | von Willebrand Factor (vWF) | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.5 ml; 300 tests |
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P180-1 | negative control | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.0 ml; 200 tests |
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P190-1 | negative control | polyclonal | Rat IgG | FITC | FC, IHC | 1.0 ml; 200 tests |
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P190-2 | negative control | polyclonal | Rat IgG | PE | FC, IHC | 1.0 ml; 200 tests |
| ||||||
P190-3 | negative control | polyclonal | Rat IgG | DyLight 649 | FC, IHC | 1.0 ml; 200 tests |
| ||||||
R300 | Antibodies for Mouse Plaet Depletion | polyclonal anti-GPIb alpha | Rat IgG | purif. | plaet depletion | 0.5 mg |
| ||||||
X488 | Antibodies for In Vivo Mouse Plaet Labeling | anti - GPIbbeta derivative | Rat IgG | DyLight 488 | in vivo | 100 µg |
| ||||||
X649 | Antibodies for In Vivo Mouse Plaet Labeling | anti - GPIbbeta derivative | Rat IgG | DyLight 649 | in vivo | 100 µg |
貨號 | 抗原類型 | 克隆方式 | 表型 | 產(chǎn)品形式 | 應(yīng)用 | 規(guī)格 | ||
C301 | 清除小鼠血小板 | polyclonal non-immune rat immunoglobulins | Rat IgG | 純化 | 清除小鼠血小板 | 0.5 mg | ||
D200 | 雙色分析血小板活性 | Wug.E9 JON/A | Rat IgG2b/IgG1 | FITC、PE | 流式細(xì)胞術(shù) | 2 x 1.0 ml; 200 tests | ||
M011-0 | GPVI | JAQ1 | Rat IgG2a | 純化 | IP, IHC, WB | 0.1 mg | ||
M011-1 | GPVI | JAQ1 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M021-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.H4 | Rat IgG2b | purif. | IP, IHC, block | 0.5 mg | ||
M021-1 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.H4 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M022-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.A1 | Rat IgG1 | purif. | IP, IHC | 0.5 mg | ||
M023-2 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | JON/A | Rat IgG2b | PE | FC (activ. form) | 1.5 ml; 300 tests | ||
M024-0 | Integrin alphaIIb (GPIIb, CD41) | MwReg30 | Rat IgG1 | purif. | IP, IHC, generation of Fab fragments | 6 mg | ||
M025-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | purif. | IP, IHC | 0.5 mg | ||
M025-1 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M025-2 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | PE | FC, IHC | 1.5 ml; 300 tests | ||
M025-3 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | DyLight 649 | FC, IHC | 1.5 ml; 300 tests | ||
M030-0 | Integrin beta3 chain (GPIIIa, CD61) | Luc.A5 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg | ||
M031-0 | Integrin beta3 chain (GPIIIa, CD61) | Luc.H11 | Rat IgG2b | purif. | IP, IHC, WB | 0.5 mg | ||
M031-1 | Integrin beta3 chain (GPIIIa, CD61) | Luc.H11 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M040-0 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | purif. | IP, IHC | 0.5 mg | ||
M040-1 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M040-2 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | PE | FC, IHC | 1.5 ml; 300 tests | ||
M040-3 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | DyLight 649 | FC, IHC | 1.5 ml; 300 tests | ||
M041-0 | GPIbalpha (CD42b) | Xia.H10 | Rat IgG2a | purif. | FC, IHC | 0.5 mg | ||
M041-1 | GPIbalpha (CD42b) | Xia.H10 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M042-0 | GPIbalpha (CD42b) | Xia.G7 | Rat IgG1 | purif. | IP, IHC, WB | 0.5 mg | ||
M042-1 | GPIbalpha (CD42b) | Xia.G7 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M043-0 | GPIbalpha (CD42b) | Xia.B2 | Rat IgG2a | purif. | IP, IHC, block | 0.5 mg | ||
M043-1 | GPIbalpha (CD42b) | Xia.B2 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M050-0 | GPIbbeta (CD42c) | Xia.C3 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg | ||
M050-1 | GPIbbeta (CD42c) | Xia.C3 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M051-0 | GPIX (CD42a) | Xia.B4 | Rat IgG1 | purif. | IP, IHC | 0.5 mg | ||
| ||||||||
M051-1 | GPIX (CD42a) | Xia.B4 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M060-1 | GPV (CD42d) | Gon.C2 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M061-1 | GPV (CD42d) | Gon.G6 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M070-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.G4 | Rat IgG2b | purif. | IP, IHC, block | 0.5 mg | ||
M070-1 | Integrin alpha2 chain (CD49b, GPIa) | Sam.G4 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M071-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1 | Rat IgG2b | purif. | IP, IHC | 0.5 mg | ||
M071-1 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M075-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1, Sam.G4, Sam.H9 | Rat IgG2b/IgG1 | purif. | IP, IHC | 0.5 mg | ||
M080-0 | Integrin alpha5 chain (CD49e) | Tap.A12 | Rat IgG1 | purif. | IP, IHC | 0.5 mg | ||
M080-1 | Integrin alpha5 chain (CD49e) | Tap.A12 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M110-0 | CD9 | Nyn.H3 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg | ||
M110-1 | CD9 | Nyn.H3 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M120-0 | CD31 (PECAM-1) | Pec.H3 | Rat IgG1 | purif. | IP, IHC, WB | 0.5 mg | ||
M130-0 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | purif. | IP, IHC | 0.5 mg | ||
M130-1 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M130-2 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | PE | FC, IHC | 1.5 ml; 300 tests | ||
P140-1 | fibrinogen | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.5 ml; 300 tests | ||
P150-1 | von Willebrand Factor (vWF) | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.5 ml; 300 tests | ||
P180-1 | negative control | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.0 ml; 200 tests | ||
P190-1 | negative control | polyclonal | Rat IgG | FITC | FC, IHC | 1.0 ml; 200 tests | ||
P190-2 | negative control | polyclonal | Rat IgG | PE | FC, IHC | 1.0 ml; 200 tests | ||
| ||||||||
P190-3 | negative control | polyclonal | Rat IgG | DyLight 649 | FC, IHC | 1.0 ml; 200 tests | ||
R300 | Antibodies for Mouse Plaet Depletion | polyclonal anti-GPIb alpha | Rat IgG | purif. | plaet depletion | 0.5 mg | ||
X488 | Antibodies for In Vivo Mouse Plaet Labeling | anti - GPIbbeta derivative | Rat IgG | DyLight 488 | in vivo | 100 µg | ||
X649 | Antibodies for In Vivo Mouse Plaet Labeling | anti - GPIbbeta derivative | Rat IgG | DyLight 649 | in vivo | 100 µg | ||
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